Self-Assembly of Geometry-Based DNA Origami-Histone Protein Hybrid Nanostructures for Constructing Rationally-Designed Higher-Order Structures

The emergence of hybrid DNA–protein nanostructures in recent years has expanded the application DNA nanotechnology. Previous studies reported integration proteins into by sequence-dependent interactions or chemical modifications DNA, which limit design flexibility nanostructures. Here we report construction sequence-independent geometry-based using self-assembly single-stranded (ss) and histone proteins. We demonstrate that nucleosome-like ssDNA-histone complexes are integrated various shapes origami at specific predefined locations. further show possibility these as building blocks for designing more complex Our finding would facilitate development predesigned flexible straightforward higher-order structures.